How to Use a Spectrophotometer

The Neuhaus Science Projects Guide, How to Use a Spectrophotometer, is written for the average high school student in grades 9 through 12.

This guide booklet is written to help students succeed with science projects that require the use of a spectrophotometer. It provides details on the use of the equipment without going into the technical details behind the operations. Preparation of solutions, setups, and procedures necessary to obtain the data needed are detailed. Colored images, graphs, and other illustrations augment the text.

Topics discussed:

How to Use a Spectrophotometer:--

How Much Is in It? Light and Color The Choice of Instrument Old and New Models The Guts of a Spectrophotometer How the System Works Guts and All Transmittance The Analytical Wavelength How to Find the Analytical Wavelength of a Substance Generic Operating Instructions The Importance of the Blank Plastic Versus Glass Matching Cuvettes Sample Concentration.

Quantitative Analysis:--

Understanding the Standard Curve Preparation of Solutions The Setup Procedure Graphing the Standard Curve How to Use the Standard Curve.

Protein Analysis:--

Preparation of Solutions The Setup Procedure Testing an Unknown Protein Sample Cleaning Up Other Methods of Protein Assay.

The Filter Colorimeter

References

Dettagli